Abstract
The sequence-specific DNA-binding protein factor F6, which binds upstream of the cluster of the chicken alpha-globin genes, has previously been found to interact with a DNA fragment containing a replication origin and a nuclear matrix binding site. This protein has been partially characterized. Based on its molecular weight and binding affinity, F6 belongs to a family of GATA proteins, the chicken equivalent of transcription factor NFE-1. An oligonucleotide including the binding site for F6 competes for binding of the above-mentioned DNA fragment to the nuclear matrix. This indicates an involvement of this protein in the interaction between DNA and the nuclear matrix.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, Nuclear
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Base Sequence
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Cell Line, Transformed
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Chickens
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DNA-Binding Proteins / drug effects
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DNA-Binding Proteins / isolation & purification
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DNA-Binding Proteins / physiology*
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Distamycins / pharmacology
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Erythroid-Specific DNA-Binding Factors
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GATA1 Transcription Factor
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Molecular Sequence Data
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Nuclear Matrix / chemistry*
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Nuclear Proteins / drug effects
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Nuclear Proteins / isolation & purification
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Nuclear Proteins / physiology*
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Regulatory Sequences, Nucleic Acid
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Transcription Factors
Substances
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Antigens, Nuclear
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DNA-Binding Proteins
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Distamycins
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Erythroid-Specific DNA-Binding Factors
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GATA1 Transcription Factor
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Nuclear Proteins
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Transcription Factors
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stallimycin