An open reading frame in the approximately 28-kb tox-argk gene cluster encodes a polypeptide with homology to fatty acid desaturases

E Hatziloukas; N J Panopoulos; S Delis; D E Prosen; N W Schaad

doi:10.1016/0378-1119(95)00569-5

An open reading frame in the approximately 28-kb tox-argk gene cluster encodes a polypeptide with homology to fatty acid desaturases

Gene. 1995 Dec 1;166(1):83-7. doi: 10.1016/0378-1119(95)00569-5.

Authors

E Hatziloukas¹, N J Panopoulos, S Delis, D E Prosen, N W Schaad

Affiliation

¹ Department of Environmental Science, Policy and Management, University of California, Berkeley 94720, USA.

PMID: 8529898
DOI: 10.1016/0378-1119(95)00569-5

Abstract

Part of an apparent open reading frame in the tox-argK gene cluster of Pseudomonas syringae pathovar phaseolicola (Psp) potentially encodes a polypeptide with sequence similarity to fatty acid desaturases (DES). Escherichia coli B expressing this segment under T7 promoter control produced a 34-kDa polypeptide. The possible involvement of a DES in facilitating phaseolotoxin (Ptx) secretion at the low temperatures normally required for its synthesis and the evolutionary implications about the origin of the tox-argK gene cluster are discussed.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Amino Acid Sequence
Base Sequence
DNA Primers / chemistry
DNA, Bacterial / genetics
Exotoxins / metabolism
Fatty Acid Desaturases / genetics
Genes, Bacterial*
Molecular Sequence Data
Multigene Family
Open Reading Frames
Ornithine / analogs & derivatives
Plant Diseases / microbiology
Pseudomonas / genetics*
Restriction Mapping
Sequence Alignment
Sequence Homology, Amino Acid
Solubility

Substances

DNA Primers
DNA, Bacterial
Exotoxins
phaseolotoxin
Ornithine
Fatty Acid Desaturases

Associated data

GENBANK/L35553