Abstract
We report that expressing interfering mutants of the small Ras-related GTPase Rac, using either recombinant vaccinia virus or stable DNA transfection, eliminates epidermal growth factor-induced Ca2+ signaling, without affecting Ca2+ mobilization or influx from G protein-coupled receptors. Platelet-derived growth factor-dependent Ca2+ influx, however, is only partly sensitive to dominant negative Rac proteins. Thus, whereas epidermal growth factor-induced Ca2+ influx is completely mediated by Rac proteins, platelet-derived growth factor-induced Ca2+ influx involves Rac-dependent and -independent signaling pathways.
MeSH terms
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Animals
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Calcium / physiology*
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Cells, Cultured
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Epidermal Growth Factor / physiology
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ErbB Receptors / physiology*
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GTP-Binding Proteins / physiology*
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HeLa Cells
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Histamine / pharmacology
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Humans
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Platelet-Derived Growth Factor / physiology
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Rats
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Receptors, Platelet-Derived Growth Factor / physiology*
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Recombinant Proteins
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Signal Transduction
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Vaccinia virus
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rac GTP-Binding Proteins
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rho GTP-Binding Proteins
Substances
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Platelet-Derived Growth Factor
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Recombinant Proteins
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Epidermal Growth Factor
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Histamine
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ErbB Receptors
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Receptors, Platelet-Derived Growth Factor
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GTP-Binding Proteins
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rac GTP-Binding Proteins
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rho GTP-Binding Proteins
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Calcium