The data presented here show that labyrinthine and facial branchiomotor efferent cells in the chicken and the mouse become postmitotic overlappingly, both spatially and temporally. Differential migration of labyrinthine efferents and facial motoneurons leads to the already described distinct distribution of labyrinthine efferents and facial motoneurons in adult brains. Differences exist between the chicken and the mouse with respect to the origin of labyrinthine efferents (rhombomere 4 and 5 for the chicken; rhombomere 4 alone for the mouse) and the way contralateral labyrinthine efferents form (migration across the floor plate in the chicken; extension of an axon across the floor plate in the mouse). The different routes taken by migrating motoneurons may all be mediated by substances released from the floor plate, some of which were recently characterized. Labyrinthine efferent axons and facial motoneuron axons segregate at distinctly different areas in the chicken and mouse: outside the brain in the former and inside the brain in the latter. Examination of the possible basis for pathway selection tends to support the idea that efferents use intact afferent fibers as highways for their navigation to distinct sensory epithelia.