Abstract
Dystrophin and alpha- and gamma-sarcoglycans were newly expressed in BC3H1 cells during differentiation induced by serum withdrawal. These proteins formed a tight complex with other dystophin-associated proteins (DAPs), as detected by immunoprecipitation with anti-dystrophin antibody. Integrins beta 1 and beta 3, vinculin, and focal adhesion kinase were also detected in the same immunoprecipitate. In a cell adhesion assay, differentiated BC3H1 cells attached more efficiently to type I collagen-coated dishes than nondifferentiated cells and loss of alpha-sarcoglycan induced by antisense ODN in differentiated cells resulted in significant inhibition of cell adhesion. Thus dystrophin and DAPs, at least partly, form a complex with the focal adhesion proteins in differentiated BC3H1 cells and alpha-sarcoglycan seems to modulate the function of the focal adhesion complex in these cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Binding Sites
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Blood
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Cell Adhesion Molecules / analysis
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Cell Differentiation
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Cell Line
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Culture Media, Serum-Free
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Cytoskeletal Proteins / biosynthesis
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Cytoskeletal Proteins / isolation & purification
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Cytoskeletal Proteins / metabolism*
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Dystrophin / biosynthesis
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Dystrophin / isolation & purification
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Dystrophin / metabolism*
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Electrophoresis, Polyacrylamide Gel
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Focal Adhesion Kinase 1
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Focal Adhesion Protein-Tyrosine Kinases
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Immunoblotting
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Integrins / analysis
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Kinetics
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Membrane Glycoproteins / biosynthesis
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Membrane Glycoproteins / isolation & purification
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Membrane Glycoproteins / metabolism*
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Mice
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Molecular Sequence Data
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Oligonucleotides, Antisense / pharmacology
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Protein Binding
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Protein-Tyrosine Kinases / analysis
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Sarcoglycans
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Vinculin / analysis
Substances
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Cell Adhesion Molecules
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Culture Media, Serum-Free
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Cytoskeletal Proteins
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Dystrophin
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Integrins
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Membrane Glycoproteins
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Oligonucleotides, Antisense
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Sarcoglycans
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Vinculin
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Protein-Tyrosine Kinases
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Focal Adhesion Kinase 1
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Focal Adhesion Protein-Tyrosine Kinases
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Ptk2 protein, mouse