Abstract
The construction of vectors for use in Schizosaccharomyces pombe using the his3+ gene as a selectable marker is described. In addition, we report the construction of a genomic library in a his3(+)-containing shuttle vector to facilitate the cloning of genes by complementation of mutant function in strains defective for His3 activity.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Cloning, Molecular
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DNA, Recombinant
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Gene Library*
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Genetic Complementation Test
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Genetic Markers
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Genetic Variation
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Genetic Vectors / genetics*
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Hydro-Lyases / genetics*
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Plasmids
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Schizosaccharomyces / genetics*
Substances
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DNA, Recombinant
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Genetic Markers
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Hydro-Lyases
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imidazoleglycerolphosphate dehydratase