Abstract
The met protooncogene was activated by a rearrangement involving the fusion of tpr (1q25) and met (7q21-31) gene sequence in a human osteosarcoma cell line (HOS) incubated in vitro with N-methyl-N-nitro-N-nitrosoguanidine (MNNG). We examined the expression of tpr-met mRNA by means of the reverse transcription-nested polymerase chain reaction (RT-nested PCR) in human two gastric cell lines (MKN-1 and MKN-45), T-cell acute lymphocytic leukemia cell line (MOLT-4), and in gastric tissue samples including normal mucosa, intestinal metaplasia and carcinoma from three surgical specimens. A DNA fragment of 88-bp was amplified in MKN-1 and MOLT-4, 96-bp in MKN-45 and of 58-bp in all nine tissue samples including gastric carcinomas. The amplified DNA sequences were not homologous with the rearranged tpr-met gene. Our study indicated that rearranged tpr-met mRNA is not expressed either in human gastric carcinoma cell lines or in gastric mucosa and carcinoma.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenocarcinoma / genetics
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Adenocarcinoma / metabolism
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Base Sequence
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Carcinoma, Papillary / genetics
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Carcinoma, Papillary / metabolism
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Carcinoma, Signet Ring Cell / genetics
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Carcinoma, Signet Ring Cell / metabolism
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Gastric Mucosa / metabolism*
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Humans
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Molecular Sequence Data
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Nuclear Pore Complex Proteins
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Polymerase Chain Reaction / methods
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins / metabolism*
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Proto-Oncogene Proteins c-met
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RNA, Messenger / genetics
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RNA, Messenger / metabolism*
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RNA, Neoplasm / genetics
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RNA, Neoplasm / metabolism*
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Receptor Protein-Tyrosine Kinases / genetics
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Receptor Protein-Tyrosine Kinases / metabolism*
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism*
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Stomach Neoplasms / genetics*
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Stomach Neoplasms / metabolism
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Tumor Cells, Cultured
Substances
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Nuclear Pore Complex Proteins
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Proto-Oncogene Proteins
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RNA, Messenger
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RNA, Neoplasm
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Recombinant Fusion Proteins
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TPR protein, human
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Proto-Oncogene Proteins c-met
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Receptor Protein-Tyrosine Kinases