Identification of novel growth-related genes linked to the mitogenic effect of PACAP on the rat pancreatic acinar cell line, AR4-2J

Ann N Y Acad Sci. 1996 Dec 26:805:760-7. doi: 10.1111/j.1749-6632.1996.tb17554.x.

Abstract

The mRNA differential display technique was employed in order to identify novel growth-related genes linked to the mitogenic effect of PACAP on AR4-2J cells. Hereby, three differentially expressed and PACAP-inducible genes were found, one of these being homologous to a recently discovered putative growth-related early response gene in mice. In AR4-2J cells, mRNA levels of this novel rat gene are increased by PACAP in a rapid and transient fashion. This effect was enhanced by cycloheximide, whereas actinomycin D prevented the stimulatory effect of PACAP.

MeSH terms

  • Animals
  • Blotting, Northern
  • Cell Division / drug effects*
  • Cell Line
  • Cycloheximide / pharmacology
  • DNA Primers
  • DNA, Complementary
  • Dactinomycin / pharmacology
  • Mice
  • Neuropeptides / pharmacology*
  • Neurotransmitter Agents / pharmacology
  • Pancreas
  • Pituitary Adenylate Cyclase-Activating Polypeptide
  • RNA, Messenger / biosynthesis*
  • Rats
  • Transcription, Genetic / drug effects*

Substances

  • Adcyap1 protein, mouse
  • Adcyap1 protein, rat
  • DNA Primers
  • DNA, Complementary
  • Neuropeptides
  • Neurotransmitter Agents
  • Pituitary Adenylate Cyclase-Activating Polypeptide
  • RNA, Messenger
  • Dactinomycin
  • Cycloheximide