Sequence and characterization of two Arabidopsis thaliana cDNAs isolated by functional complementation of a yeast gln3 gdh1 mutant

FEBS Lett. 1997 Jun 30;410(2-3):213-8. doi: 10.1016/s0014-5793(97)00590-5.

Abstract

We have isolated two Arabidopsis thaliana cDNAs by complementation of a yeast gln3 gdh1 strain that is affected in the regulation of nitrogen metabolism. The two clones (RGA1 and RGA2) are homologous to each other and to the SCARECROW (SCR) gene that is involved in regulating an asymmetric cell division in plants. RGA1, RGA2 and SCR share several structural features and may define a new family of genes. RGA1 and RGA2 have been mapped, respectively, to chromosome II and I, and their expression in plant is constitutive.

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / genetics*
  • Base Sequence
  • DNA, Complementary
  • DNA, Plant*
  • DNA-Binding Proteins / genetics*
  • Fungal Proteins / genetics*
  • Genetic Complementation Test
  • Glutamate Dehydrogenase / genetics*
  • Molecular Sequence Data
  • Mutation
  • Repressor Proteins*
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins*
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Transcription Factors*

Substances

  • DNA, Complementary
  • DNA, Plant
  • DNA-Binding Proteins
  • Fungal Proteins
  • GLN3 protein, S cerevisiae
  • Repressor Proteins
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Glutamate Dehydrogenase

Associated data

  • GENBANK/Y11336
  • GENBANK/Y11337