Polymorphonuclear leukocyte lysosomal proteases, cathepsins B and D affect the fibrinolytic system in human umbilical vein endothelial cells

Y Kimura; K Yokoi-Hayashi

doi:10.1016/0167-4889(95)00161-1

Polymorphonuclear leukocyte lysosomal proteases, cathepsins B and D affect the fibrinolytic system in human umbilical vein endothelial cells

Biochim Biophys Acta. 1996 Jan 10;1310(1):1-4. doi: 10.1016/0167-4889(95)00161-1.

Authors

Y Kimura¹, K Yokoi-Hayashi

Affiliation

¹ New Drug Research Department, High Quality-Life Research Laboratories, Sumitomo Metal Industries, Souraku-gun, Kyoto, Japan.

PMID: 9244167
DOI: 10.1016/0167-4889(95)00161-1

Abstract

To clarify the physiological role played by neutrophil lysosomal protease in cultured human umbilical vein endothelial cells (HUVEC), we studied the effects of cathepsins B and D released from activated polymorphonuclear leukocytes on the fibrinolytic system in HUVEC. Cathepsins B and D reduced the antigens of tissue-type plasminogen activator, and they increased both the antigens and the activity of plasminogen activator inhibitor-1. These results suggest that cathepsins B and D are involved in the thrombotic tendency, since they inhibited the fibrinolytic system in cultured HUVEC.

MeSH terms

Cathepsin B / pharmacology
Cathepsin B / physiology*
Cathepsin D / pharmacology
Cathepsin D / physiology*
Cells, Cultured / drug effects
Endothelium, Vascular / physiology*
Fibrinolysis / drug effects
Humans
Lysosomes / enzymology
Neutrophils / enzymology*
Plasminogen Activator Inhibitor 1 / analysis
Tissue Plasminogen Activator / analysis

Substances

Plasminogen Activator Inhibitor 1
Tissue Plasminogen Activator
Cathepsin B
Cathepsin D