Cell-free ubiquitination of cell cycle regulators in budding yeast extracts

MeSH terms

• Anaphase-Promoting Complex-Cyclosome
• Cell Cycle*
• Cell-Free System
• Chromatography, DEAE-Cellulose
• Cloning, Molecular / methods
• Cyclin-Dependent Kinase Inhibitor Proteins
• Cyclins / genetics
• Enzyme Inhibitors / isolation & purification
• Enzyme Inhibitors / metabolism
• Escherichia coli
• Fungal Proteins / isolation & purification
• Fungal Proteins / metabolism*
• Ligases / biosynthesis
• Ligases / isolation & purification
• Ligases / metabolism*
• Mutagenesis, Site-Directed
• Point Mutation
• Polymerase Chain Reaction / methods
• Recombinant Proteins / biosynthesis
• Recombinant Proteins / isolation & purification
• Recombinant Proteins / metabolism
• Saccharomyces cerevisiae / cytology
• Saccharomyces cerevisiae / genetics
• Saccharomyces cerevisiae / physiology*
• Saccharomyces cerevisiae Proteins*
• Spheroplasts / physiology
• Templates, Genetic
• Transcription, Genetic
• Ubiquitin-Protein Ligase Complexes*
• Ubiquitin-Protein Ligases
• Ubiquitins / metabolism*