Positioning of 72 potentially full size LTRs of human endogenous retroviruses HERV-K on the human chromosome 19 map. Occurrences of the LTRs in human gene sites

Gene. 1997 Oct 15;199(1-2):255-64. doi: 10.1016/s0378-1119(97)00376-4.

Abstract

Seventy-two near full size long terminal repeats (LTRs) of human endogenous retrovirus of K-family (HERV-K) have been precisely located on the metric map of human chromosome 19. The LTR-related sequences were identified and assigned to cosmids by hybridization with two independent chromosome 19 specific cDNA clones corresponding to different parts of U3 region of LTR of HERV-K. The presence of full-size LTR sequences in a cosmid was further verified by PCR assay with a pair of primers complementary to the termini of the LTR. Coincidences of the LTR and the known genes positions are discussed.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Base Sequence
  • Chromosome Mapping*
  • Chromosomes, Human, Pair 19 / genetics*
  • Chromosomes, Human, Pair 19 / virology
  • Cosmids / genetics
  • Genes / genetics
  • Humans
  • Molecular Sequence Data
  • Repetitive Sequences, Nucleic Acid / genetics*
  • Retroviridae / genetics*
  • Sequence Homology, Nucleic Acid