Abstract
To improve the quality of cytoplasmic labelling of GAL4-expressing cells in Drosophila enhancer-trap and transgenic strains, a new GAL4-responsive reporter UAS-tau, which features a bovine tau cDNA under control of a yeast upstream activation sequence (UAS), was tested. Tau, a microtubule-associated protein, is distributed actively and evenly into all cellular processes. Monoclonal anti-bovine Tau antibody reveals the axonal structure of the labelled cells with detail similar to that of Golgi impregnation. We demonstrate that the UAS-tau system is especially useful for studying processes of differentiation and reorganisation of identified neurones during postembryonic development.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Genetically Modified
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Antibodies, Monoclonal
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Cattle
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Central Nervous System / anatomy & histology
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Central Nervous System / cytology
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Central Nervous System / growth & development
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DNA-Binding Proteins / genetics
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Drosophila / anatomy & histology
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Drosophila / cytology
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Drosophila / growth & development*
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Enhancer Elements, Genetic / physiology
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Fungal Proteins / genetics*
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Gene Expression Regulation, Developmental / physiology
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Genes, Reporter
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Genetic Testing / methods
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Metamorphosis, Biological / physiology
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Neurons / physiology
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Saccharomyces cerevisiae Proteins*
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Transcription Factors*
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tau Proteins / genetics*
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tau Proteins / immunology
Substances
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Antibodies, Monoclonal
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DNA-Binding Proteins
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Fungal Proteins
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GAL4 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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tau Proteins