TCRV and TCRJ gene usage in MBP responding T cells from (B10.PL x PL/J)F1 mice is biased towards that of B10.PL mice

J Neuroimmunol. 1997 Dec;80(1-2):13-22. doi: 10.1016/s0165-5728(97)00129-x.

Abstract

We analyzed myelin basic protein (MBP) specific T cell hybridoma clones from (B10.PL x PL/J)F1 mice. MBP-reacting T cell hybridomas from F1 mice preferentially expressed B10.PL TcraV2.3 (53%) and B10.PL TcraV4.2 (13%) with minor expression of TcraV4.4 (13%) gene segments. A dominant expression of TcrbV8.2 (73%) accompanying with TcrbV8.1 (20%) and TcrbV13 (7%) gene segments have been identified in these MBP-reacting T cell hybridomas from F1 mice. There was less restrictive but non-random usage of the TcraJ and TcrbJ gene segments. Overall, the MBP-reacting T cell hybridomas from (B10.PL x PL/J)F1 mice were dominated by the MBP-reacting T cell pattern seen in B10.PL mice.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Crosses, Genetic
  • Flow Cytometry
  • Gene Expression Regulation / immunology
  • Gene Rearrangement, T-Lymphocyte / immunology*
  • Genes, T-Cell Receptor / immunology*
  • Hybridomas
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Multigene Family
  • Myelin Basic Protein / genetics*
  • Myelin Basic Protein / immunology
  • Myelin Basic Protein / metabolism
  • Rats
  • Receptors, Antigen, T-Cell, alpha-beta / biosynthesis
  • Receptors, Antigen, T-Cell, alpha-beta / genetics
  • Species Specificity
  • T-Lymphocytes / metabolism*

Substances

  • Myelin Basic Protein
  • Receptors, Antigen, T-Cell, alpha-beta