The insertion and deletion polymorphisms of region V of mitochondrial DNA (mtDNA) were studied in three indigenous populations of northern Asia: Yakuts, Evens, and Koryaks. In Evens and Koryaks, polymorphism was not found. In Yakuts, a 9-bp deletion and a 4-bp insertion were revealed in mtDNA region V. Sequencing of mtDNA region V confirmed the presence of these mutations. The results obtained, together with data published earlier, indicate that the frequencies of both the deletion and the insertion in Yakuts are 1.21%. The association between the 9-bp deletion and the types of the mtDNA control region were studied. The data obtained indicated that this deletion belonged to the Asian deletion cluster.