Abstract
A cDNA encoding the human bifunctional ATP sulfurylase/adenosine 5'-phosphosulfate (APS) kinase was cloned and sequenced. The enzyme contains an APS kinase domain in its N-terminal portion and an ATP sulfurylase domain in its C-terminal portion. Recombinant full-length enzyme and its constituent APS kinase and ATP sulfurylase domains were individually expressed, purified, and shown to have their respective enzymatic activities.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular*
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Cross-Linking Reagents / metabolism
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DNA Primers
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Gene Expression Regulation, Enzymologic*
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Humans
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Molecular Sequence Data
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Phosphotransferases (Alcohol Group Acceptor) / biosynthesis
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Phosphotransferases (Alcohol Group Acceptor) / genetics
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Phosphotransferases (Alcohol Group Acceptor) / physiology*
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Sulfate Adenylyltransferase / biosynthesis
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Sulfate Adenylyltransferase / genetics
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Sulfate Adenylyltransferase / physiology*
Substances
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Cross-Linking Reagents
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DNA Primers
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Phosphotransferases (Alcohol Group Acceptor)
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adenylylsulfate kinase
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Sulfate Adenylyltransferase