Abstract
This report describes the identification of a cDNA encoding STK13, a third human protein kinase related to the Drosophila Aurora and the budding yeast Ipl1 kinases. After screening of a human placental cDNA library with a Xenopus laevis cDNA encoding the pEg2 protein kinase and 5' RACE on testis mRNA, a full-length cDNA was isolated. The chromosomal localization of STK13 on 19q13.3-ter between the markers D19S210 and D19S218 was established by a combination of somatic cell and radiation hybrid panel PCR screening. The localization of STK13 on human chromosome 19 was confirmed by fluorescence in situ hybridization (FISH) using a genomic clone containing STK13 as a probe.
Copyright 1998 Academic Press.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Aurora Kinase C
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Aurora Kinases
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Base Sequence
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Chromosome Mapping
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Chromosomes, Human, Pair 19 / genetics*
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Cloning, Molecular
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DNA Primers / genetics
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DNA, Complementary / genetics
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DNA, Complementary / isolation & purification
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Drosophila / genetics
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Female
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Humans
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In Situ Hybridization, Fluorescence
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Intracellular Signaling Peptides and Proteins
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Male
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Molecular Sequence Data
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Pregnancy
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Protein Kinases / genetics*
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Protein Serine-Threonine Kinases / genetics*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae Proteins*
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Xenopus laevis / genetics
Substances
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DNA Primers
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DNA, Complementary
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Intracellular Signaling Peptides and Proteins
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Saccharomyces cerevisiae Proteins
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Protein Kinases
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AURKC protein, human
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Aurora Kinase C
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Aurora Kinases
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IPL1 protein, S cerevisiae
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Protein Serine-Threonine Kinases
Associated data
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GDB/9837399
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GENBANK/AF059681
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GENBANK/AF059682