Invertase activity associated with the walls of Solanum tuberosum tubers

Phytochemistry. 1999 Feb;50(4):525-34. doi: 10.1016/s0031-9422(98)00474-9.


Three fractions with invertase activity (beta-D-fructofuranoside fructohydrolase, EC were isolated from mature Solanum tuberosum tubers: acid soluble invertase, invertase I and invertase II. The first two invertases were purified until electrophoretic homogeneity. They are made by two subunits with an apparent M(r) value of 35,000 and their optimal pH is 4.5. Invertase I was eluted from cell walls with ionic strength while invertase II remained tightly bound to cell walls after this treatment. This invertase was solubilized by enzymatic cell wall degradation (solubilized invertase II). Their K(m)s are 28, 20, 133 and 128 mM for acid soluble invertase, invertase I, invertase II and solubilized invertase II, respectively. Glucose is a non-competitive inhibitor of invertase activities and fructose produces a two site competitive inhibition with interaction between the sites. Bovine serum albumin produces activation of the acid soluble invertase and invertase I while a similar inhibition by lectins and endogenous proteinaceous inhibitor from mature S. tuberosum tubers was found. Invertase II (tightly bound to the cell walls) shows a different inhibition pattern. The test for reassociation of the acid soluble invertase or invertase I on cell wall, free of invertase activity, caused the reappearance of all invertase forms with their respective solubilization characteristics and molecular and kinetic properties. The invertase elution pattern, the recovery of cell wall firmly bound invertase and the coincidence in the immunological recognition, suggest that all three invertases may be originated from the same enzyme. The difference in some properties of invertase II and solubilized invertase II from the other two enzymes would be a consequence of the enzyme microenvironment in the cell wall or the result of its wall binding.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies / immunology
  • Blotting, Western
  • Catalysis
  • Cell Wall / enzymology*
  • Enzyme Inhibitors / metabolism
  • Glycoside Hydrolases / antagonists & inhibitors
  • Glycoside Hydrolases / isolation & purification
  • Glycoside Hydrolases / metabolism*
  • Hydrogen-Ion Concentration
  • Kinetics
  • Lectins / metabolism
  • Plant Lectins
  • Solanum tuberosum / enzymology*
  • Substrate Specificity
  • beta-Fructofuranosidase


  • Antibodies
  • Enzyme Inhibitors
  • Lectins
  • Plant Lectins
  • Glycoside Hydrolases
  • beta-Fructofuranosidase