Human glomerular endothelial cells IGFBPs are regulated by IGF-I and TGF-beta1

Mol Cell Endocrinol. 1999 Aug 20;154(1-2):123-36. doi: 10.1016/s0303-7207(99)00063-5.


The release of insulin-like growth factor binding proteins (IGFBPs) and their regulation in human glomerular endothelial cells (GENC) was characterised. GENC produce IGFBP-4, IGFBP-2 and IGFBP-3 and express mRNA for IGFBP-2 to IGFBP-5. Due to the fact that IGF-I and TGF-beta1 modulate glomerular hypertrophy, their action on IGFBP release and GENC growth was studied. IGF-I increased IGFBP-3, IGFBP-2 and decreased IGFBP-4, while TGF-beta1 decreased IGFBP-3 and apparently increased IGFBP-4. All of the IGFBPs, except the TGF-beta1-regulated IGFBP-4, were modulated at mRNA level. IGF-I stimulated GENC proliferation, while TGF-beta1 inhibited their growth. It was demonstrated that an IGFBP-3 antibody reduced GENC proliferation. However, rhIGFBP-3 alone had no effect on GENC, but after 48 h pre-incubation the IGF-I stimulated GENC growth was increased, suggesting that IGFBP-3 could modulate the IGF-I induced GENC proliferation. It was concluded that the stimulatory IGFBP-3 and the inhibitory IGFBP-4 could regulate GENC growth, although the IGFBP-3 seems to have a predominant effect in this control.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Division / drug effects
  • Culture Media, Conditioned / chemistry
  • Endothelium / chemistry*
  • Endothelium / cytology
  • Gene Expression Regulation / drug effects
  • Humans
  • Insulin-Like Growth Factor Binding Proteins / drug effects*
  • Insulin-Like Growth Factor Binding Proteins / genetics
  • Insulin-Like Growth Factor I / genetics
  • Insulin-Like Growth Factor I / metabolism
  • Insulin-Like Growth Factor I / pharmacology*
  • Kidney Glomerulus / cytology*
  • RNA, Messenger / metabolism
  • Transforming Growth Factor beta / genetics
  • Transforming Growth Factor beta / metabolism
  • Transforming Growth Factor beta / pharmacology*


  • Culture Media, Conditioned
  • Insulin-Like Growth Factor Binding Proteins
  • RNA, Messenger
  • Transforming Growth Factor beta
  • Insulin-Like Growth Factor I