Retinoic acid delays transcription of human retinal pigment neuroepithelium marker genes in ARPE-19 cells

Neuroreport. 2000 May 15;11(7):1571-9.


The effect of retinoic acid on the differentiation of a human retinal pigment epithelium-derived cell line ARPE-19 was studied. Differentiation of ARPE-19 cells is delayed by retinoic acid. The minimum all-trans-retinoic acid concentration needed for delay of ARPE-19 differentiation is 1 microM. A delay of differentiation was also observed using 1 microM 9-cis or 13-cis-retinoic acid. Differentiation at the molecular level was studied by analyzing transcription of two RPE-marker genes, RPE65 and peropsin. In the presence of 1 microM retinoic acid the onset of transcription of both genes was delayed by 2-3 weeks. We conclude that all-trans-, 9-cis-, and 13-cis-retinoic acid delay differentiation of ARPE-19 cells into cells that phenotypically resemble cells from the human retinal pigment epithelium.

MeSH terms

  • Actins / genetics
  • Antineoplastic Agents / pharmacology*
  • Biomarkers
  • Carrier Proteins
  • Cell Differentiation / drug effects
  • Cells, Cultured
  • Eye Proteins / genetics
  • Gene Expression / drug effects
  • Humans
  • Pigment Epithelium of Eye / cytology
  • Pigment Epithelium of Eye / drug effects
  • Pigment Epithelium of Eye / physiology*
  • Proteins / genetics
  • RNA, Messenger / analysis
  • Rhodopsin
  • Transcription, Genetic / drug effects*
  • Tretinoin / pharmacology*
  • cis-trans-Isomerases


  • Actins
  • Antineoplastic Agents
  • Biomarkers
  • Carrier Proteins
  • Eye Proteins
  • Proteins
  • RNA, Messenger
  • RRH protein, human
  • Tretinoin
  • Rhodopsin
  • retinoid isomerohydrolase
  • cis-trans-Isomerases