Apoptosis induced by the flavonoid from lemon fruit (Citrus limon BURM. f.) and its metabolites in HL-60 cells

Biosci Biotechnol Biochem. 2000 May;64(5):1075-8. doi: 10.1271/bbb.64.1075.

Abstract

The flavonoid from lemon fruit (Citrus limon BURM. f.) and its metabolites, particularly eriodictyol, 3,4-dihydroxyhydrocinnamic acid, and phloroglucinol had the function of DNA fragmentation in HL-60 cells when analyzed by flow cytometry. An apoptotic DNA ladder and chromatin condensation were observed in HL-60 cells when treated with these compounds. The caspase inhibitor prevented DNA fragmentation. These compounds are anticipated to be useful for medical purposes.

MeSH terms

  • Apoptosis / drug effects
  • Apoptosis / physiology*
  • Caffeic Acids / pharmacology
  • Caspase Inhibitors
  • Cell Size
  • Citrus / chemistry*
  • DNA Fragmentation / drug effects
  • Dose-Response Relationship, Drug
  • Electrophoresis, Agar Gel
  • Flavanones*
  • Flavonoids / chemistry
  • Flavonoids / metabolism*
  • Flavonoids / pharmacology*
  • HL-60 Cells
  • Hesperidin / analogs & derivatives*
  • Hesperidin / pharmacology
  • Humans
  • Phloroglucinol / pharmacology

Substances

  • Caffeic Acids
  • Caspase Inhibitors
  • Flavanones
  • Flavonoids
  • eriocitrin
  • Phloroglucinol
  • Hesperidin
  • eriodictyol
  • caffeic acid