Characterization of the 5'-flanking region of the gene encoding the 50 kDa subunit of human DNA polymerase delta

Biochim Biophys Acta. 2000 Sep 7;1493(1-2):231-6. doi: 10.1016/s0167-4781(00)00153-6.


DNA polymerase delta consists of at least four subunits: p125, p68, p50, and p12 [Liu et al., J. Biol. Chem. 275 (2000) 18739-18744]. We have isolated genomic DNA clones covering the gene for the human DNA polymerase delta 50 kDa subunit (POLD2) and its 5'-flanking sequence. The POLD2 gene is composed of 11 exons and is distributed over 10 kb of genomic DNA. All exon-intron splice junctions conformed to the GT/AG consensus sequence. The 5'-flanking region of human POLD2 is G+C-rich and does not have a typical TATA box. A computer-based search for potential transcription factor binding sites revealed the existence of a number of motifs including those for AP1, AP2, Sp1, NF-1 and CREB. The functional activity of the regulatory region of the human POLD2 gene was demonstrated by its ability to drive the expression of a chloramphenicol acetyltransferase reporter gene in COS-7 cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Binding Sites
  • COS Cells
  • Cloning, Molecular
  • DNA Polymerase III / chemistry
  • DNA Polymerase III / genetics*
  • Genes, Reporter
  • Humans
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • Restriction Mapping
  • Transcription Factors / chemistry


  • Transcription Factors
  • DNA Polymerase III