A highly sensitive and specific enzyme-linked immunosorbent assay of antibodies to hepatitis C virus

Acta Virol. 2000 Feb;44(1):29-33.


In this study, a 178 amino acids long portion of the hepatitis C virus (HCV) core gene was cloned, sequenced, expressed in Escherichia coli, and purified. The resulting antigen (C178) was tested with human sera enzyme-linked immunosorbent assay (ELISA) in order to assess its ability to diagnose HCV. It was shown by ELISA that 92% of the patients sera, diagnosed previously by a 3rd generation enzyme immunoassay (EIA) as HCV-positive, had antibodies against the C178 antigen. This antigen gave no false positive results when tested with anti-HCV-negative sera.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cloning, Molecular
  • DNA, Viral / biosynthesis
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Escherichia coli / genetics
  • Hepacivirus / immunology
  • Hepatitis C / virology*
  • Hepatitis C Antibodies / blood*
  • Hepatitis C Antigens / biosynthesis
  • Hepatitis C Antigens / immunology*
  • Humans
  • Recombinant Proteins / biosynthesis
  • Sensitivity and Specificity
  • Serologic Tests
  • Viral Core Proteins / biosynthesis
  • Viral Core Proteins / genetics


  • DNA, Viral
  • Hepatitis C Antibodies
  • Hepatitis C Antigens
  • Recombinant Proteins
  • Viral Core Proteins