Inhibition of androgen receptor-mediated transcription by amino-terminal enhancer of split

Mol Cell Biol. 2001 Jul;21(14):4614-25. doi: 10.1128/MCB.21.14.4614-4625.2001.


A yeast two-hybrid assay has identified an androgen-dependent interaction of androgen receptor (AR) with amino-terminal enhancer of split (AES), a member of the highly conserved Groucho/TLE family of corepressors. Full-length AR, as well as the N-terminal fragment of AR, showed direct interactions with AES in in vitro protein-protein interaction assays. AES specifically inhibited AR-mediated transcription in a well-defined cell-free transcription system and interacted specifically with the basal transcription factor (TFIIE) in HeLa nuclear extract. These observations implicate AES as a selective repressor of ligand-dependent AR-mediated transcription that acts by directly interacting with AR and by targeting the basal transcription machinery.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Androgen Receptor Antagonists
  • Co-Repressor Proteins
  • Epithelial Cells / cytology
  • Gene Expression Regulation*
  • HeLa Cells
  • Humans
  • Male
  • Prostate / cytology
  • Proteins*
  • Receptors, Androgen / genetics
  • Receptors, Androgen / metabolism*
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism*
  • Transcription Factors / genetics
  • Transcription Factors / physiology
  • Transcription Factors, TFII*
  • Transcription, Genetic


  • Androgen Receptor Antagonists
  • Co-Repressor Proteins
  • Proteins
  • Receptors, Androgen
  • Repressor Proteins
  • TLE5 protein, human
  • Transcription Factors
  • Transcription Factors, TFII
  • transcription factor TFIIE