DNA polymerase V-dependent mutator activity in an SOS-induced Escherichia coli strain with a temperature-sensitive DNA polymerase III

Mutat Res. 2002 Jan 29;499(1):97-101. doi: 10.1016/s0027-5107(01)00267-6.

Abstract

The temperature-sensitive DNA polymerase III (Pol III) encoded by the dnaE486 allele confers a spontaneous mutator activity in SOS-induced bacteria that is largely dependent upon DNA polymerase V (Pol V), encoded by umuD, C. This mutator activity is influenced by the defective proof-reading sub-unit of Pol III encoded by the dnaQ905 (mutD5) allele arguing that Pol V is most likely fixing mutations arising from mismatched primer termini produced by Pol III(486). The size of the dnaQ effect is, however, modest leaving open the possibility that Pol V may be responsible for some of the mutator effect by engaging in bursts of processive activity.

MeSH terms

  • DNA Polymerase III / genetics*
  • DNA Polymerase III / metabolism
  • DNA-Directed DNA Polymerase / physiology*
  • Escherichia coli / physiology*
  • Escherichia coli Proteins / genetics
  • Exodeoxyribonuclease V
  • Exodeoxyribonucleases / genetics
  • Mutation*
  • SOS Response, Genetics
  • Temperature

Substances

  • Escherichia coli Proteins
  • UmuC protein, E coli
  • DNA polymerase III, alpha subunit
  • DNA Polymerase III
  • DNA polymerase V, E coli
  • DNA-Directed DNA Polymerase
  • UmuD protein, E coli
  • dnaQ protein, E coli
  • Exodeoxyribonucleases
  • Exodeoxyribonuclease V