An NMR approach to structural proteomics

Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):1825-30. doi: 10.1073/pnas.042684599.


The influx of genomic sequence information has led to the concept of structural proteomics, the determination of protein structures on a genome-wide scale. Here we describe an approach to structural proteomics of small proteins using NMR spectroscopy. Over 500 small proteins from several organisms were cloned, expressed, purified, and evaluated by NMR. Although there was variability among proteomes, overall 20% of these proteins were found to be readily amenable to NMR structure determination. NMR sample preparation was centralized in one facility, and a distributive approach was used for NMR data collection and analysis. Twelve structures are reported here as part of this approach, which allowed us to infer putative functions for several conserved hypothetical proteins.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins / chemistry
  • Databases as Topic
  • Escherichia coli / metabolism
  • Magnetic Resonance Spectroscopy / methods*
  • Models, Molecular
  • Open Reading Frames
  • Proteins / chemistry*
  • Software
  • Species Specificity


  • Bacterial Proteins
  • Proteins

Associated data

  • PDB/1IIO
  • PDB/1ILO
  • PDB/1IQO
  • PDB/1JDQ
  • PDB/1JE3
  • PDB/1JJG
  • PDB/1JRM
  • PDB/1JSB
  • PDB/1JW2
  • PDB/1JW3
  • PDB/1JYG