Multiple regulatory roles of a novel Saccharomyces cerevisiae protein, encoded by YOL002c, in lipid and phosphate metabolism

J Biol Chem. 2002 May 31;277(22):19609-17. doi: 10.1074/jbc.M202045200. Epub 2002 Mar 26.


The yeast open reading frame YOL002c encodes a putative membrane protein. This protein is evolutionarily conserved across species, including humans, although the function of each of these proteins remains unknown. YOL002c is highly expressed in yeast cells that are grown in the presence of saturated fatty acids such as myristate. Furthermore, cells in which the YOL002c gene is disrupted grow poorly on this carbon source. These mutant cells are also resistant to the polyene antibiotic, nystatin. Gene chip analysis on yol002cDelta cells revealed that a variety of genes encoding proteins involved in fatty acid metabolism and in the phosphate signaling pathway are induced in this mutant strain. In addition, our studies demonstrated that in the disruption strain acid phosphatase activity is expressed constitutively, and the cells accumulate polyphosphate to much higher levels than wild-type cells. A homologous human protein is able to partially rescue these defects in phosphate metabolism. We propose that YOL002c encodes a Saccharomyces cerevisiae protein that plays a key role in metabolic pathways that regulate lipid and phosphate metabolism.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Antifungal Agents / pharmacology
  • Blotting, Northern
  • Cell Membrane / metabolism
  • Conserved Sequence
  • DNA / metabolism
  • DNA Primers / pharmacology
  • Fatty Acids / metabolism
  • Humans
  • Lipid Metabolism
  • Lipids / chemistry*
  • Membrane Proteins / chemistry*
  • Membrane Proteins / metabolism*
  • Molecular Sequence Data
  • Mutation
  • Nystatin / pharmacology
  • Oligonucleotide Array Sequence Analysis
  • Open Reading Frames
  • Phenotype
  • Phosphates / chemistry*
  • Phosphates / metabolism
  • Plasmids / metabolism
  • Polyphosphates / chemistry
  • Polyphosphates / metabolism
  • Promoter Regions, Genetic
  • RNA / metabolism
  • Recombination, Genetic
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / chemistry*
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Sequence Homology, Amino Acid
  • Signal Transduction
  • beta-Galactosidase / metabolism


  • Antifungal Agents
  • DNA Primers
  • Fatty Acids
  • Lipids
  • Membrane Proteins
  • Phosphates
  • Polyphosphates
  • Saccharomyces cerevisiae Proteins
  • YOL002c protein, S cerevisiae
  • Nystatin
  • RNA
  • DNA
  • beta-Galactosidase