The high-speed Hydra-Plus-One system for automated high-throughput protein crystallography

Acta Crystallogr D Biol Crystallogr. 2002 Oct;58(Pt 10 Pt 1):1523-6. doi: 10.1107/s090744490201435x. Epub 2002 Sep 26.


An automated high-throughput dispenser has been developed for the setup of protein crystallization trials by vapor diffusion or Microbatch methods. The Hydra-Plus-One is composed of a Hydra-PP system equipped with a motorized XYZ-platform, 96 precision glass syringes and a single-channel microsolenoid dispenser, which transfers 100 nl-50 micro l of protein solution with an accuracy of > 90% at a speed of 60s per 96 wells. Up to 300 micro l of premixed cocktails can be aspirated with the 96-syringe-assembly and dispensed into reservoir and droplet wells within 60s. The Hydra-Plus-One combines high precision, reliability and speed in a cost-effective high-throughput system ideally suited for protein crystallization

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins / chemistry
  • Crystallography / instrumentation*
  • Crystallography / methods
  • Crystallography / statistics & numerical data
  • Mycobacterium tuberculosis / chemistry
  • Proteins / chemistry*
  • Reproducibility of Results


  • Bacterial Proteins
  • Proteins