Fast mapping of protein-protein interfaces by NMR spectroscopy

J Am Chem Soc. 2003 Nov 26;125(47):14250-1. doi: 10.1021/ja037640x.


Identifying the interface of protein complexes can represent a difficult task in structural biology. Here, we report a method for the fast mapping of interfaces of protein complexes by NMR without the need for the assignments of the proteins involved.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Binding Sites
  • Nerve Tissue Proteins / chemistry*
  • Nerve Tissue Proteins / metabolism
  • Nitric Oxide Synthase / chemistry*
  • Nitric Oxide Synthase / metabolism
  • Nitric Oxide Synthase Type I
  • Nuclear Magnetic Resonance, Biomolecular / methods
  • Peptide Mapping / methods
  • Protein Conformation
  • Protein Structure, Tertiary


  • Nerve Tissue Proteins
  • postsynaptic density proteins
  • Nitric Oxide Synthase
  • Nitric Oxide Synthase Type I