Isolation and molecular characterization of multiresistant Staphylococcus sciuri and Staphylococcus haemolyticus associated with skin and soft-tissue infections

J Med Microbiol. 2004 Jan;53(Pt 1):51-55. doi: 10.1099/jmm.0.05294-0.

Abstract

The isolation, molecular identification and genotyping of multiresistant Staphylococcus sciuri and Staphylococcus haemolyticus from skin and soft-tissue infections are reported. Accurate and full identification of three coagulase-negative staphylococcal isolates was achieved using PCR, while the API STAPH method failed to identify an isolate of S. haemolyticus fully. The PCR assay, which detects polymorphism in the 16S-23S rRNA spacer region, is shown to be potentially useful for rapid and accurate identification of coagulase-negative staphylococci. Identical PFGE type and antibiotic-resistance profiles of two methicillin-resistant S. haemolyticus isolates in this study suggest the existence of a multiresistant community clone.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Child
  • DNA Fingerprinting
  • DNA, Bacterial / analysis
  • DNA, Bacterial / chemistry
  • Drug Resistance, Multiple, Bacterial
  • Electrophoresis, Gel, Pulsed-Field
  • Female
  • Humans
  • Male
  • Microbial Sensitivity Tests
  • Phenotype
  • Soft Tissue Infections / microbiology*
  • Staphylococcal Infections / microbiology*
  • Staphylococcal Skin Infections / microbiology*
  • Staphylococcus / classification
  • Staphylococcus / drug effects
  • Staphylococcus / genetics
  • Staphylococcus / isolation & purification*
  • Staphylococcus haemolyticus / classification
  • Staphylococcus haemolyticus / drug effects
  • Staphylococcus haemolyticus / genetics
  • Staphylococcus haemolyticus / isolation & purification*
  • Wound Infection / microbiology*

Substances

  • DNA, Bacterial