Comparison of cell viability on anorganic bone matrix with or without P-15 cell binding peptide

Biomaterials. 2004 Aug;25(19):4831-6. doi: 10.1016/j.biomaterials.2003.12.007.


Cell-extracellular cell matrix (ECM) interactions play a vital role in development including cytoskeletal structure, growth and differentiation. A 15 amino acid, non-RGD containing, peptide that is derived from type I collagen was tested for its ability to confer cell survival. Two routinely utilized bone graft materials, anorganic bone matrix (ABM) and ABM carrying the cell binding peptide P-15 were compared for cell viability and apoptosis. Anchorage-dependent human foreskin fibroblasts (HFF) or osteogenic MC3T3-E1 cells were seeded on ABM or ABM/P-15. After serum withdrawal, viability and level of apoptosis were significantly (p<0.05) improved for cells on ABM/P-15 compared to cells on ABM. Scanning electron microscopy confirmed large, spread cells on ABM/P-15 and smaller cells with apoptotic cellular blebs on ABM. In addition, viable cell attachment was significantly greater on cells cultured on ABM/P-15 compared with demineralized freeze-dried bone allograft. This is the first report that the P-15 cell binding peptide, when complexed with ABM, promotes cell survival. The P-15 peptide may modulate cell number and tissue structure by the enhancement of viable cell attachment and via the regulation of apoptosis.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • 3T3 Cells
  • Animals
  • Biomimetic Materials / chemistry
  • Biomimetic Materials / pharmacology
  • Bone Substitutes / chemistry*
  • Bone Transplantation / methods
  • Cell Adhesion / drug effects
  • Cell Adhesion / physiology
  • Cell Survival / drug effects
  • Cell Survival / physiology
  • Collagen / chemistry*
  • Collagen / pharmacology*
  • Extracellular Matrix / chemistry*
  • Fibroblasts / cytology*
  • Fibroblasts / drug effects
  • Fibroblasts / physiology*
  • Humans
  • Inorganic Chemicals / chemistry
  • Materials Testing
  • Mice
  • Peptide Fragments / chemistry*
  • Peptide Fragments / pharmacology*
  • Tissue Engineering / methods*


  • Bone Substitutes
  • Inorganic Chemicals
  • Peptide Fragments
  • cell-binding peptide P-15
  • Collagen