Heterologous human immunodeficiency virus type 1 priming-boosting immunization strategies involving replication-defective adenovirus and poxvirus vaccine vectors

J Virol. 2004 Oct;78(20):11434-8. doi: 10.1128/JVI.78.20.11434-11438.2004.


We compared the human immunodeficiency virus type 1 (HIV-1)-specific cellular immune responses elicited in nonhuman primates by HIV-1 gag-expressing replication-defective adenovirus serotype 5 (Ad5) or poxvirus vectors, used either alone or in combination with each other. The responses arising from a heterologous Ad5 priming-poxvirus boosting regimen were significantly greater than those elicited by homologous regimens with the individual vectors or by a heterologous poxvirus priming-Ad5 boosting regimen. The heterologous Ad5 priming-poxvirus boosting approach may have potential utility in humans as a means of inducing high levels of cellular immunity.

MeSH terms

  • AIDS Vaccines / administration & dosage
  • AIDS Vaccines / immunology*
  • Adenoviridae / genetics*
  • Adenoviridae / immunology
  • Animals
  • Gene Products, gag / immunology*
  • Genetic Vectors
  • HIV Infections / immunology
  • HIV Infections / prevention & control
  • HIV-1 / immunology
  • Humans
  • Immunization*
  • Immunization, Secondary
  • Interferon-gamma / metabolism
  • Macaca mulatta
  • Poxviridae / genetics*
  • Poxviridae / immunology
  • T-Lymphocytes / immunology*
  • Virus Replication


  • AIDS Vaccines
  • Gene Products, gag
  • Interferon-gamma