Interleukin-17F induces pulmonary neutrophilia and amplifies antigen-induced allergic response

Am J Respir Crit Care Med. 2005 Jan 1;171(1):12-8. doi: 10.1164/rccm.200406-778OC. Epub 2004 Oct 11.


Interleukin (IL)-17F is a recently described human cytokine belonging to the IL-17 gene family, but its in vivo function remains to be determined. To this end, a full-length mouse IL-17F cDNA sequence with a 483-bp coding region sequence was first identified. Pulmonary gene transfer of an IL-17F expression construct (pcDNAmIL-17F) in mice was used to investigate its regulatory role. The results showed first that a significant increase in the number of neutrophils was seen in the bronchoalveolar lavage fluids of IL-17F-transduced mice, concomitant with increased expression of genes encoding C-X-C chemokines and inflammatory cytokines when compared with mock and phosphate-buffered saline control animals. Mucosal transfer of the IL-17F gene in ovalbumin (OVA)-sensitized mice before antigen (Ag) challenge enhanced the levels of Ag-induced pulmonary neutrophilia, but not eosinophilia, goblet cell hyperplasia, and mucin gene expression. However, no significant change in the levels of Th2 cytokine expression was noted. A significant enhancement of ventilatory timing in response to inhaled methacholine was also seen in IL-17F-transduced, Ag-sensitized mice, whereas a small but significant increase was found in IL-17F-transduced, naive mice. These results suggest a role for IL-17F in the induction of neutrophilia in the lungs and in the exacerbation of Ag-induced pulmonary inflammation.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Allergens
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Bronchi / pathology
  • Bronchoalveolar Lavage Fluid / chemistry
  • Bronchoalveolar Lavage Fluid / cytology*
  • Chemokines / genetics
  • Chemokines / metabolism
  • Cytokines / genetics
  • Cytokines / metabolism
  • Female
  • Gene Expression
  • Gene Transfer Techniques
  • Inflammation
  • Interleukin-17 / chemistry
  • Interleukin-17 / genetics
  • Interleukin-17 / physiology*
  • Lung / pathology*
  • Mice
  • Mice, Inbred BALB C
  • Neutrophils / pathology*
  • Ovalbumin / immunology
  • Plethysmography, Whole Body
  • Respiration
  • Respiratory Hypersensitivity / immunology
  • Respiratory Hypersensitivity / pathology
  • Respiratory Hypersensitivity / physiopathology*
  • Transduction, Genetic
  • Up-Regulation


  • Allergens
  • Chemokines
  • Cytokines
  • Interleukin-17
  • Ovalbumin