MgSO4 and lazaroid (U-83836E) partially protects glioma cells against glutamate toxicity in vitro

Acta Neurobiol Exp (Wars). 2004;64(4):461-6. doi: 10.55782/ane-2004-1528.


In this study, the possible effects of MgSO4 and lazaroid (U-83836E) on glutamate toxicity on glial cells were investigated. C6 and human glioblastoma multiforme cells derived from two patients were grown in an incubator. First, determined IC50 dose of L-glutamate (L-glu) was given for 24 hours and removed, and then respective MgSO4 or U-83836E doses were added to the culture medium. After 24 hours 3-(4,5-Dimethylthyazol-2-yl)-2,5-diphenyltetrazolium bromide, thiazolyl blue (MTT) test was applied. When compared to the L-glu-treated group, MgSO4 at the dose of 0.01 mM induced C6 and human glioma cell growth by 17%, 15% and 5%, respectively. At the dose of 1 microM U-83836E also increased C6 and human glioma cell growth by 12%, 13% and 5%, respectively. In conclusion, although MgSO4 and U-83836E do not strongly block glutamate-induced cell death, it is suggested that reduction of Mg2+ ions and free radical production may have a role in glutamate toxicity on glial cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / pharmacology*
  • Calcium Channel Blockers / pharmacology*
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Chromans / pharmacology*
  • Dose-Response Relationship, Drug
  • Drug Interactions
  • Glioma
  • Glutamic Acid / pharmacology*
  • Humans
  • Inhibitory Concentration 50
  • Magnesium Sulfate / pharmacology*
  • Mice
  • Neuroglia / cytology
  • Neuroglia / drug effects*
  • Piperazines / pharmacology*
  • Tetrazolium Salts
  • Thiazoles


  • Antioxidants
  • Calcium Channel Blockers
  • Chromans
  • Piperazines
  • Tetrazolium Salts
  • Thiazoles
  • U 78517F
  • Glutamic Acid
  • Magnesium Sulfate
  • thiazolyl blue