A single active-site region for a group II intron

Nat Struct Mol Biol. 2005 Jul;12(7):626-7. doi: 10.1038/nsmb957. Epub 2005 Jun 26.


Despite the biological importance of self-splicing group II introns, little is known about their structural organization. Synthetic incorporation of site-specific photo-cross-linkers within catalytic domains resulted in functional distance constraints that, when combined with known tertiary interactions, provide a three-dimensional view of the active intron architecture. All functionalities important for both steps of splicing are proximal before the first step, suggestive of a single active-site region for group II intron catalysis.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Binding Sites / genetics
  • Binding Sites / physiology*
  • Catalysis
  • Cross-Linking Reagents / metabolism
  • Guanosine / analogs & derivatives
  • Introns / genetics
  • Introns / physiology*
  • Models, Molecular*
  • RNA Splicing / genetics
  • RNA Splicing / physiology*
  • Thionucleosides
  • Thiouridine


  • Cross-Linking Reagents
  • Thionucleosides
  • Guanosine
  • Thiouridine
  • 6-thioguanosine