Molecular characterization of mtDNA depleted and repleted NT2 cell lines

Mitochondrion. 2005 Aug;5(4):255-65. doi: 10.1016/j.mito.2005.04.003.


Transmitochondrial cytoplasmic hybrids (cybrids) enable functional assessment of mitochondrial DNA (mtDNA)-encoded proteins. Cybrid production often utilizes cell lines depleted of endogenous mtDNA (rho0 cells), and a number of suitable rho0 cell lines exist for this purpose. We now provide molecular data characterizing an NT2 human teratocarcinoma rho0 cell line, as well as NT2 cybrid derivatives. NT2 rho0 cells contained no detectable mtDNA on a sensitive PCR assay. Eight weeks after exogenous mtDNA transfer cybrids showed no evidence of endogenous mtDNA reversion, and heteroplasmic ratios of a single nucleotide substitution roughly reflected that of the blood samples used to repopulate their mtDNA.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Line, Tumor
  • Clone Cells
  • DNA, Mitochondrial / analysis
  • DNA, Mitochondrial / physiology*
  • Ethidium / pharmacology
  • Humans
  • Hybrid Cells / drug effects
  • Hybrid Cells / physiology*
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Teratocarcinoma


  • DNA, Mitochondrial
  • Ethidium