26-Locus Y-STR typing in a Bhutanese population sample

Forensic Sci Int. 2006 Aug 10;161(1):1-7. doi: 10.1016/j.forsciint.2005.10.008. Epub 2005 Nov 14.


26 Y chromosome short tandem repeat (STR) loci were amplified in a sample of 856 unrelated males from Bhutan, using two multiplex polymerase chain reaction (PCR) assays. The first multiplex is the Y-STR 20plex described by Butler et al. [J.M. Butler, R. Schoske, P.M. Vallone, M.C. Kline, A.J. Redd, M.F. Hammer, A novel multiplex for simultaneous amplification of 20 Y chromosome STR markers, Forensic Sci. Int. 129 (2002) 10-24], and the second is a novel (but overlapping) 14plex that targets six additional Y-STRs (DYS425, DYS434, DYS435, DYS436, DYS461, DYS462) and also amplifies the amelogenin locus. The 26-loci give a discriminating power of 0.9957, though even at this resolution one haplotype occurs 24 times. We identify novel alleles at five loci and microvariants at a further three, which were characterised by sequencing. Extended (11-locus) haplotypes for these samples have been submitted to the Y-STR Haplotype Reference Database (YHRD).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amelogenin
  • Bhutan
  • Chromosomes, Human, Y*
  • DNA Fingerprinting
  • Dental Enamel Proteins / genetics
  • Gene Frequency
  • Genetics, Population*
  • Humans
  • Male
  • Polymerase Chain Reaction
  • Tandem Repeat Sequences*


  • Amelogenin
  • Dental Enamel Proteins