The octamer binding transcription factor Oct-1 is a stress sensor

Cancer Res. 2005 Dec 1;65(23):10750-8. doi: 10.1158/0008-5472.CAN-05-2399.


The POU-domain transcription factor Oct-1 is widely expressed in adult tissues and has been proposed to regulate a large group of target genes. Microarray expression profiling was used to evaluate gene expression changes in Oct-1-deficient mouse fibroblasts. A number of genes associated with cellular stress exhibited altered expression. Consistent with this finding, Oct-1-deficient fibroblasts were hypersensitive to gamma radiation, doxorubicin, and hydrogen peroxide and harbored elevated reactive oxygen species. Expression profiling identified a second group of genes dysregulated in Oct-1-deficient fibroblasts following irradiation, including many associated with oxidative and metabolic stress. A number of these genes contain octamer sequences in their immediate 5' regulatory regions, some of which are conserved in human. These results indicate that Oct-1 modulates the activity of genes important for the cellular response to stress.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Animals
  • Doxorubicin
  • Fibroblasts / drug effects
  • Fibroblasts / radiation effects
  • Gene Expression Regulation / drug effects
  • Gene Expression Regulation / physiology*
  • Gene Expression Regulation / radiation effects
  • Hydrogen Peroxide
  • Mice
  • Octamer Transcription Factor-1 / deficiency
  • Octamer Transcription Factor-1 / genetics
  • Octamer Transcription Factor-1 / physiology*
  • Oxidative Stress
  • Reverse Transcriptase Polymerase Chain Reaction


  • Octamer Transcription Factor-1
  • Doxorubicin
  • Hydrogen Peroxide