Anti-inflammatory effect of lemon mucilage: in vivo and in vitro studies

Immunopharmacol Immunotoxicol. 2005;27(4):661-70. doi: 10.1080/08923970500418919.


The mucilage extracted from a lemon juice centrifugation pulp was studied for its anti-inflammatory effect in rat. In vivo the lemon mucilage significantly inhibited carrageenan-induced edema in rat paw from 59% to 73.5% showing the highest effect at the third hour. In vitro, at the doses of 10(-8), 10(-6), 10(-4) or 10(-2) mg/mL the lemon mucilage stimulated the superoxide anion production in rat testing neutrophils in whole blood but inhibited it in FMLP stimulated cells at the dose of 10(-2) mg/mL. The neutrophils of rats receiving p.o. the lemon mucilage for 21 days showed a significant decrease of 45.5% in O2- generation after FMLP stimulation, and a not-significant increase after phorbol-12-myristate-13-acetate (PMA) or zymosan stimulation. Since the activity on zymosan- and PMA-induced O2- production was not significant, the inhibition exerted by FMLP in rat neutrophils occurred mainly through the blockade of phospholipase D.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / administration & dosage
  • Anti-Inflammatory Agents / pharmacology*
  • Carrageenan
  • Citrus*
  • Disease Models, Animal
  • Edema / chemically induced
  • Edema / prevention & control*
  • Male
  • N-Formylmethionine Leucyl-Phenylalanine / pharmacology
  • Neutrophils / drug effects
  • Neutrophils / metabolism
  • Plant Extracts / administration & dosage
  • Plant Extracts / pharmacology*
  • Rats
  • Rats, Wistar
  • Superoxides / blood
  • Time Factors


  • Anti-Inflammatory Agents
  • Plant Extracts
  • Superoxides
  • N-Formylmethionine Leucyl-Phenylalanine
  • Carrageenan