Protective effect of panaxydol and panaxynol on sodium nitroprusside-induced apoptosis in cortical neurons

Chem Biol Interact. 2006 Apr 15;160(3):225-31. doi: 10.1016/j.cbi.2006.02.001. Epub 2006 Mar 10.


An excess of the free radical nitric oxide (NO) is viewed as a deleterious factor involved in various CNS disorders. The protective effect of panaxydol (PND) and panaxynol (PNN) on sodium nitroprusside (SNP)-induced neuronal apoptosis and potential mechanism were investigated in primary cultured rat cortical neurons. Pretreatment of the cells with PND or PNN for 24 h following 1mM SNP, an exogenous NO donor, exposure for 1h, resulted significantly in reduction of cell death induced by SNP determined by MTT assay, LDH release and Hoechst staining. 5 microM PND and PNN also reduced the up-regulation of the pro-apoptotic gene, Bax, down-regulation of the anti-apoptotic gene, Bcl-2. The observations demonstrated that PND and PNN protect neurons against SNP-induced apoptosis via regulating the apoptotic related genes. The results raise the possibility that PND and PNN reduce neurodegeneration in the Alzheimer's brain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkynes / pharmacology
  • Alzheimer Disease / metabolism
  • Alzheimer Disease / pathology
  • Animals
  • Apoptosis / drug effects*
  • Apoptosis / physiology
  • Cells, Cultured
  • Cerebral Cortex / cytology
  • Diynes
  • Fatty Alcohols / pharmacology
  • Free Radicals / metabolism
  • Neurons / drug effects*
  • Neurons / pathology
  • Nitric Oxide / metabolism
  • Nitroprusside / toxicity*
  • Protective Agents / pharmacology*
  • Rats
  • Rats, Sprague-Dawley


  • Alkynes
  • Diynes
  • Fatty Alcohols
  • Free Radicals
  • Protective Agents
  • Nitroprusside
  • falcarinol
  • Nitric Oxide
  • panaxydol