A proteome reference map and proteomic analysis of Bifidobacterium longum NCC2705

Mol Cell Proteomics. 2006 Jun;5(6):1105-18. doi: 10.1074/mcp.M500410-MCP200. Epub 2006 Mar 20.


A comprehensive proteomic study was carried out to identify and characterize proteins expressed by Bifidobacterium longum NCC2705. A total of 708 spots representing 369 protein entries were identified by MALDI-TOF-MS and/or ESI-MS/MS. Isoelectric point values estimated by gel electrophoresis matched closely with their predicted ones, although some discrepancies exist suggesting that post-translational protein modifications might be common in B. longum. The identified proteins represent 21.4% of the predicted 1727 ORFs in the genome and correspond to 30% of the predicted proteome. Moreover 95 hypothetical proteins were experimentally identified. This is the first compilation of a proteomic reference map for the important probiotic organism B. longum NCC2705. The study aimed to define a number of cellular pathways related to important physiological processes at the proteomic level. Proteomic comparison of glucose- and fructose-grown cells revealed that fructose and glucose are catabolized via the same degradation pathway. Interestingly the sugar-binding protein specific to fructose (BL0033) and Frk showed higher levels of expression in cells grown on fructose than on glucose as determined by semiquantitative RT-PCR. BL0033 time course and concentration experiments showed that the induction time and fructose concentration correlates to increased expression of BL0033. At the same time, an ABC (ATP-binding cassette) transporter ATP-binding protein (BL0034) was slightly up-regulated in cells grown on fructose compared with glucose. All of the above results suggest that the uptake of fructose into the cell may be conducted by a specific transport system in which BL0033 might play an important role.

Publication types

  • Comparative Study

MeSH terms

  • ATP-Binding Cassette Transporters
  • Amino Acids / metabolism
  • Bacterial Proteins / analysis*
  • Bifidobacterium / chemistry*
  • Bifidobacterium / genetics
  • Bifidobacterium / metabolism
  • Electrophoresis, Gel, Two-Dimensional
  • Energy Metabolism
  • Fructose / metabolism
  • Glucose / metabolism
  • Glycolysis
  • Heat-Shock Proteins / analysis
  • Nucleotides / metabolism
  • Open Reading Frames
  • Proteome / analysis*
  • Proteomics*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization


  • ATP-Binding Cassette Transporters
  • Amino Acids
  • Bacterial Proteins
  • Heat-Shock Proteins
  • Nucleotides
  • Proteome
  • Fructose
  • Glucose