CD44 and TGFbeta1 synergise to induce expression of a functional NADPH oxidase in promyelocytic cells

Biochem Biophys Res Commun. 2006 May 5;343(2):609-16. doi: 10.1016/j.bbrc.2006.03.003. Epub 2006 Mar 10.


Bone marrow stromal cells produce large amounts of extracellular matrix and cytokines. Amongst them, hyaluronan, a glycosaminoglycan and ligand for the cell surface molecule CD44, and TGFbeta1, a cytokine particularly important in monocyte differentiation. We have studied in vitro the role of hyaluronan and TGFbeta1 in the differentiation process of U937 monocytic progenitor cells. We provide evidence that, in the presence of whole blood-derived serum, the addition of hyaluronan is sufficient to induce the expression of NADPH-oxidase components but not of other monocytic markers (CD14, CD11b, and VLA-4). In the presence of plasma-derived serum, besides hyaluronan, the additional presence of TGFbeta1 was required for the expression of all of the components of the NADPH oxidase. We further show that hyaluronan mediates its effect through CD44. We conclude that cell matrix factors act cooperatively with cytokines to induce the expression of the components of the NADPH-oxidase in monocytic progenitor cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Differentiation / drug effects
  • Cell Line
  • Dose-Response Relationship, Drug
  • Drug Combinations
  • Drug Synergism
  • Gene Expression Regulation, Enzymologic / drug effects
  • Granulocyte Precursor Cells / cytology*
  • Granulocyte Precursor Cells / drug effects
  • Granulocyte Precursor Cells / enzymology*
  • Humans
  • Hyaluronan Receptors / administration & dosage*
  • Monocytes / cytology*
  • Monocytes / drug effects
  • Monocytes / enzymology*
  • NADPH Oxidases / metabolism*
  • Transforming Growth Factor beta / administration & dosage*
  • Transforming Growth Factor beta1


  • Drug Combinations
  • Hyaluronan Receptors
  • TGFB1 protein, human
  • Transforming Growth Factor beta
  • Transforming Growth Factor beta1
  • NADPH Oxidases