Abstract
The 5' terminal structure of the mRNA synthesized in vitro by the virion-associated RNA polymerase of vesicular stomatitis virus in the presence of S-adenosyl-L-methione consists of 7-methyl guanosine linked to 2'-O-methyl adenosine through a 5'-5' pyrophosphate bond as m7G(5')ppp(5')A-m-p ... The alpha and beta phosphated of GTP and alpha phosphate of ATP are incorporated into the blocked 5' terminal structure.
MeSH terms
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Adenosine / analysis
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Adenosine Triphosphate / metabolism
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Alkaline Phosphatase / metabolism
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Cell-Free System
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Chromatography, DEAE-Cellulose
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Chromatography, Paper
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DNA-Directed RNA Polymerases / metabolism
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Diphosphates / analysis
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Electrophoresis, Paper
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Guanosine / analysis
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Guanosine Triphosphate / metabolism
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Methylation
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Oligonucleotides / analysis
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Phosphoric Diester Hydrolases / metabolism
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Pyrophosphatases / metabolism
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RNA, Messenger / analysis*
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RNA, Messenger / biosynthesis
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RNA, Viral / analysis*
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RNA, Viral / biosynthesis
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Ribonucleases / metabolism
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S-Adenosylmethionine / metabolism
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Vesicular stomatitis Indiana virus / analysis*
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Vesicular stomatitis Indiana virus / enzymology
Substances
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Diphosphates
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Oligonucleotides
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RNA, Messenger
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RNA, Viral
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Guanosine
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S-Adenosylmethionine
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Guanosine Triphosphate
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Adenosine Triphosphate
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DNA-Directed RNA Polymerases
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Ribonucleases
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Alkaline Phosphatase
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Phosphoric Diester Hydrolases
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Pyrophosphatases
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Adenosine