Cloning and sequencing the urocanase gene (hutU) from Pseudomonas putida

FEBS Lett. 1991 Jul 29;286(1-2):55-7. doi: 10.1016/0014-5793(91)80938-y.

Abstract

A clone harbouring the entire urocanase gene (hutU) was obtained from a genomic library of Pseudomonas putida using oligonucleotide probes synthesised on the basis of known flanking sequences. One subunit of urocanase consists of 556 amino acids and has a molecular mass of 60,771 Da.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA, Bacterial
  • Genes, Bacterial
  • Histidine / metabolism
  • Molecular Sequence Data
  • Multigene Family
  • Polymerase Chain Reaction
  • Pseudomonas / enzymology
  • Pseudomonas / genetics*
  • Urocanate Hydratase / genetics*

Substances

  • DNA, Bacterial
  • Histidine
  • Urocanate Hydratase

Associated data

  • GENBANK/M95211
  • GENBANK/X58483
  • GENBANK/X58797
  • GENBANK/X58798
  • GENBANK/X58799
  • GENBANK/X58800
  • GENBANK/X58801
  • GENBANK/X58802
  • GENBANK/X58803
  • GENBANK/X62841