Stimulation of noradrenaline release in the cerebral cortex via presynaptic N-methyl-D-aspartate (NMDA) receptors and their pharmacological characterization

J Neural Transm Suppl. 1991:34:121-7. doi: 10.1007/978-3-7091-9175-0_16.

Abstract

In rat brain cortex synaptosomes superfused with Mg(2+)-free solution containing glycine, [3H]noradrenaline (3H-NA) release evoked by NMDA was abolished by omission of glycine or Ca2+ and inhibited by Mg2+, the competitive and noncompetitive NMDA receptor antagonists CGP 37849 and dizocilpine (MK-801), respectively, as well as by ethanol, but was not affected by tetrodotoxin. The 3H-NA release evoked by L-glutamate was also competitively inhibited by CGP 37849. In conclusion, presynaptic NMDA receptors mediate stimulation of NA release. Ethanol probably acts at this receptor system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 2-Amino-5-phosphonovalerate / analogs & derivatives
  • 2-Amino-5-phosphonovalerate / pharmacology
  • Animals
  • Calcium / pharmacology
  • Cerebral Cortex / metabolism*
  • Dizocilpine Maleate / pharmacology
  • Glutamates / pharmacology
  • Glutamic Acid
  • Glycine / pharmacology
  • Kinetics
  • Magnesium / pharmacology
  • Male
  • N-Methylaspartate / pharmacology*
  • Norepinephrine / metabolism*
  • Rats
  • Rats, Inbred Strains
  • Receptors, N-Methyl-D-Aspartate / antagonists & inhibitors
  • Receptors, N-Methyl-D-Aspartate / drug effects
  • Receptors, N-Methyl-D-Aspartate / physiology*
  • Synaptosomes / drug effects
  • Synaptosomes / metabolism*

Substances

  • Glutamates
  • Receptors, N-Methyl-D-Aspartate
  • 2-amino-4-methyl-5-phosphono-3-pentenoic acid
  • Glutamic Acid
  • N-Methylaspartate
  • Dizocilpine Maleate
  • 2-Amino-5-phosphonovalerate
  • Magnesium
  • Calcium
  • Glycine
  • Norepinephrine