Plasmodium yoelii yoelii 17XNL constitutively expressing GFP throughout the life cycle

Exp Parasitol. 2007 Mar;115(3):310-3. doi: 10.1016/j.exppara.2006.09.008. Epub 2006 Oct 17.


Plasmodium yoelii is a rodent parasite commonly used as a model to study malaria infection. It is the preferred model parasite for liver-stage immunological studies and is also widely used to study hepatocyte, erythrocyte and mosquito infection. We have generated a P. yoelii yoelii 17XNL line that is stably transfected with the green fluorescent protein (gfp) gene. This parasite line constitutively expresses high levels of GFP during the complete parasite life cycle including liver, blood and mosquito stages. These fluorescent parasites can be used in combination with fluorescence activated cell sorting or live microscopy for a wide range of experimental applications.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Anopheles / parasitology
  • Antimalarials / pharmacology
  • Disease Models, Animal
  • Drug Resistance
  • Erythrocytes / parasitology
  • Flow Cytometry
  • Gene Expression Regulation, Developmental* / drug effects
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism*
  • Insect Vectors / parasitology
  • Life Cycle Stages / genetics
  • Life Cycle Stages / physiology*
  • Liver / parasitology
  • Malaria / parasitology*
  • Mice
  • Plasmodium yoelii / drug effects
  • Plasmodium yoelii / genetics
  • Plasmodium yoelii / growth & development
  • Plasmodium yoelii / metabolism*
  • Pyrimethamine / pharmacology
  • Transfection


  • Antimalarials
  • Green Fluorescent Proteins
  • Pyrimethamine