A method for detection of human papillomavirus DNA in stored slides of stained cervical smears

Pathol Res Pract. 1990 Oct;186(5):666-7. doi: 10.1016/S0344-0338(11)80231-1.


A simple method was developed for extraction of DNA from stored slides of Papanicolaou stained cervical smears. HPV type 16 DNA was detected by DNA hybridization in 7/10 samples from known HPV-16 positive patients, whereas the 10 samples from negative patients were also negative with our assay. Using our method for obtaining data from stained cervical smears, it will be possible to design and perform historical cohort studies on stored material.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Viral / analysis*
  • Female
  • Humans
  • Nucleic Acid Hybridization*
  • Papanicolaou Test*
  • Papillomaviridae / genetics*
  • Preservation, Biological*
  • Staining and Labeling
  • Vaginal Smears*


  • DNA, Viral