Thirty years of Escherichia coli DNA gyrase: from in vivo function to single-molecule mechanism

Biochimie. 2007 Apr;89(4):490-9. doi: 10.1016/j.biochi.2007.02.012. Epub 2007 Feb 24.


The level of negative DNA supercoiling of the Escherichia coli chromosome is tightly regulated in the cell and influences many DNA metabolic processes including DNA replication, transcription, repair and recombination. Gyrase is the only type II topoisomerase able to introduce negative supercoils into DNA, a unique ability that arises from the specialized C-terminal DNA wrapping domain of the GyrA subunit. Here, we review the biological roles of gyrase in vivo and its mechanism in vitro.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Adenosine Diphosphate / metabolism
  • Adenosine Triphosphate / metabolism
  • Binding Sites
  • Catalysis
  • DNA Gyrase / chemistry
  • DNA Gyrase / metabolism*
  • DNA, Bacterial / genetics
  • DNA, Bacterial / metabolism
  • Escherichia coli / enzymology*
  • Escherichia coli Proteins / chemistry
  • Escherichia coli Proteins / metabolism
  • Models, Molecular
  • Protein Conformation


  • DNA, Bacterial
  • Escherichia coli Proteins
  • Adenosine Diphosphate
  • Adenosine Triphosphate
  • DNA Gyrase