Antioxidant effect of the marine algae Chlorella vulgaris against naphthalene-induced oxidative stress in the albino rats

Mol Cell Biochem. 2007 Sep;303(1-2):39-44. doi: 10.1007/s11010-007-9453-2. Epub 2007 Apr 25.


Alcoholic extract of the marine algae Chlorella vulgaris was examined for its free radical scavenging effect with reference to naphthalene-induced lipid peroxidation in serum, liver, and kidney of rats. Initially, upon naphthalene intoxication (435 mg/kg body weight, intraperitoneally), the lipid peroxidation activity increased significantly (P < 0.001), and in contrast, the enzymic antioxidants (superoxide dismutase, catalase, glutathione peroxidase) and non-enzymic antioxidants (glutathione, ascorbic acid, and alpha-tocopherol) levels decreased remarkably. When the naphthalene stressed rats were treated with Chlorella vulgaris extract (70 mg/kg body weight, orally), the lipid peroxidation activity reduced significantly (P < 0.001) and the activities of both the enzymic and non-enzymic antioxidants increased reaching near control values. The minimum concentration (70 mg/l) of the extract that exhibited maximum (85%) free radical scavenging activity was chosen for the experimental study. The present results suggest that Chlorella vulgaris extract exerts its chemo-preventive effect by modulating the antioxidants status and lipid peroxidation during naphthalene intoxication.

MeSH terms

  • Animals
  • Antioxidants / pharmacology*
  • Ascorbic Acid / pharmacology
  • Catalase / metabolism
  • Chlorella vulgaris / chemistry*
  • Free Radical Scavengers / metabolism
  • Glutathione / metabolism
  • Glutathione Peroxidase / metabolism
  • Lipid Peroxidation / drug effects
  • Male
  • Naphthalenes / pharmacology*
  • Oxidative Stress / drug effects*
  • Plant Extracts / pharmacology*
  • Rats
  • Rats, Wistar
  • Reactive Oxygen Species / metabolism
  • Superoxide Dismutase / metabolism
  • Thiobarbituric Acid Reactive Substances / metabolism
  • Vitamin E / metabolism


  • Antioxidants
  • Free Radical Scavengers
  • Naphthalenes
  • Plant Extracts
  • Reactive Oxygen Species
  • Thiobarbituric Acid Reactive Substances
  • Vitamin E
  • naphthalene
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Glutathione
  • Ascorbic Acid