A low-cost method for brain slice cultures

J Pharmacol Sci. 2007 Jun;104(2):191-4. doi: 10.1254/jphs.sc0070119. Epub 2007 Jun 8.


Low-cost, simple procedures for organotypic tissue cultures are desirable for high-throughput biological experiments such as large-scale medical/drug screening. We present a practical and economical method to cultivate brain slices using hydrophilic filtration membranes. With a cost reduction of more than 90%, this technique allows us to prepare hippocampal slice cultures that are morphologically and functionally indistinguishable from those obtained by the widely used Millicell-CM method.

MeSH terms

  • Animals
  • Cost Savings*
  • Hippocampus* / drug effects
  • Hippocampus* / pathology
  • Hippocampus* / physiology
  • Models, Biological*
  • Organ Culture Techniques / economics
  • Organ Culture Techniques / methods*
  • Rats
  • Rats, Sprague-Dawley
  • Rats, Wistar
  • Staining and Labeling